Journal
BRITISH JOURNAL OF DERMATOLOGY
Volume 148, Issue 2, Pages 334-336Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-2133.2003.05070.x
Keywords
angiogenesis; biostimulation; low level laser; microangiopathy
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Background Endothelial cell (EC) proliferation plays a key role in the process of tissue repair. Low-intensity laser irradiation has been demonstrated to accelerate wound healing and to improve microvascularization. Objectives The present study evaluated a possible stimulatory influence of low-intensity laser irradiation on human umbilical vein endothelial cell (HUVEC) proliferation in a systematic manner. Methods Subconfluent cultures of HUVEC were irradiated every other day with a 670-nm diode laser (intensity: 10-65 mW cm(-2) , dose: 2-8 J cm(-2) ) during a period of 6 days. Cell proliferation was evaluated quantitatively by counting in a haemocytometer. Results Our data demonstrate a dose-dependent and intensity-dependent stimulatory effect of laser irradiation on HUVEC cell proliferation. Doses of between 2 and 8 J cm(-2) induced statistically significant cell proliferation. Testing different intensities at a constant dose of 8 J cm(-2) , 20 and 65 mW cm(-2) induced most pronounced cell proliferation. Conclusions Low-intensity laser irradiation influences EC proliferation and might thereby contribute to the increase in angiogenesis and the acceleration of wound healing in vivo .
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