Journal
JOURNAL OF CELL BIOLOGY
Volume 160, Issue 3, Pages 399-407Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200212057
Keywords
actin stress fibers; lamellipodia; Rac1; actin nucleation; Arp2/3
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Funding
- NCI NIH HHS [CA90498, R01 CA090498] Funding Source: Medline
- NIGMS NIH HHS [GM26338, R01 GM026338] Funding Source: Medline
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Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein encoded by a gene that is down-regulated in transformed cells. EPLIN increases the number and size of actin stress fibers and inhibits membrane ruffling induced by Rac. EPLIN has at least two actin binding sites. Purified recombinant EPLIN inhibits actin filament depolymerization and cross-links filaments in bundles. EPLIN does not affect the kinetics of spontaneous actin polymerization or elongation at the barbed end, but inhibits branching nucleation of actin filaments by Arp2/3 complex. Side binding activity may stabilize filaments and account for the inhibition of nucleation mediated by Arp2/3 complex. We propose that EPLIN promotes the formation of stable actin filament structures such as stress fibers at the expense of more dynamic actin filament structures such as membrane ruffles. Reduced expression of EPLIN may contribute to the motility of invasive tumor cells.
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