Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 31, Issue 1, Pages 29-38Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0731-7085(02)00599-X
Keywords
cyproheptadine hydrochloride; cyproheptadine chloromethochloride; dichloromethane adduct; LC-MS; NMR; TLC
Categories
Ask authors/readers for more resources
The British Pharmacopoeia (BP) monograph for cyproheptadine HCl tablets requires a 'Related substances' thin-layer chromatography (TLC) test. This test revealed an extraneous spot with an R-f of 0.1 in certain cyproheptadine HCl tablets that were under ambient retention conditions as well as those on stability programs. An investigation utilizing LC-MS, direct infusion MS, NMR, and organic synthesis has identified that the spot results from the N-oxide of cyproheptadine (a genuine degradate) and a co-eluting cyproheptadine-dichloromethane adduct, an artifact formed during the sample extraction step in which dichloromethane is used in the extracting solvent. (C) 2002 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available