Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 987, Issue 1-2, Pages 103-109Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)02048-4
Keywords
molecular imprinting; solid-phase extraction; hyoscyamine; scopolamine; tropane alkaloids; belladonna alkaloids; alkaloids
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Molecularly imprinted polymers (MIPs) selective for scopolamine were produced using hyoscyamine (a close structural analogue) as template molecule. The produced polymers were used as media for solid-phase extraction, exhibiting selective binding properties for the analyte from biological samples. Human and calf urine and serum were processed on the MIP under various extraction protocols. The best performance was observed after loading the analyte in aqueous environment facilitating retention on the MIP by non-selective hydrophobic interactions. The MIPs were subsequently washed using an optimised solvent system to enable selective desorption of the analyte. Other related and non-related compounds were accessed to evaluate molecular recognition properties. Recoveries of up to 79% were achieved for the analyte of interest from biological samples. (C) 2002 Elsevier Science B.V. All rights reserved.
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