Journal
GENES & DEVELOPMENT
Volume 17, Issue 4, Pages 438-442Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1064703
Keywords
siRNA; miRNA; RNAi; translational repression; 3 ' UTR
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Funding
- NCI NIH HHS [P30-CA14051, P01 CA042063, P30 CA014051, P01-CA42063] Funding Source: Medline
- NIGMS NIH HHS [R37-GM34277, R37 GM034277] Funding Source: Medline
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With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA (siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3' UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs.
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