Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 21, Issue 4-6, Pages 239-252Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1381-1177(02)00229-1
Keywords
beta-galactosidase; chemical grafting; biocatalytic membranes; nylon membranes; isothermal bioreactors
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beta-Galactosidase was immobilized on nylon/poly(glycidyl methacrylate) membranes through spacers of different length: hexamethylenediamine, ethylenediamine or hydrazine. The effect of the spacer length on the catalytic behavior of the three membranes was studied in isothermal bioreactors. The behavior of the soluble and insoluble enzymes was compared to know the effects of the immobilization process and of the spacer length. The enzyme derivatives in comparison with the soluble enzyme exhibited shifts of the optimum pH values towards more acidic solutions. These shifts were found decreasing with the spacer length; while an opposite trend was observed when the optimum temperature values were considered. Also the values of the apparent K-m were found to decrease with the spacer length. All these results indicated that a soluble enzyme could be considered as an enzyme immobilized on a solid support through a spacer of infinite length. (C) 2002 Elsevier Science B.V. All rights reserved.
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