Journal
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
Volume 14, Issue 11, Pages 6949-6953Publisher
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2013.14.11.6949
Keywords
Helenalin; Telomerase; real time quantitative PCR; beta-cyclodextrin
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Funding
- Drug Applied Research Center of Tabriz University of Medical Sciences
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Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in beta-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. beta-Cyclodextrin (beta-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared beta-cyclodextrin-helenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assay showed that not only beta-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that beta-cyclodextrin-helenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of beta-cyclodextrin-helenalin complexes increased. Conclusions: beta-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, beta-cyclodextrin could be superior carrier for this kind of hydrophobic agent.
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