Journal
FEMS MICROBIOLOGY LETTERS
Volume 219, Issue 2, Pages 241-248Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1097(03)00050-8
Keywords
Legionella pneumophila; regulation; stationary phase; amoeba
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In order to identify a potential regulator of virulence gene expression in Legionella pneumophila, the L. pneumophila homologue of the response regulator GacA, LetA, was identified and cloned, facilitating the generation of a L. pneumophila letA insertion mutant. The L. pneumophila letA insertion mutant was more sensitive to oxidative and acid stress than the wild-type. The letA mutant exhibited reduced infectivity and was defective for intracellular growth within Acanthamoeba castellanii. Transcription of the rpoS and dotA genes was reduced in the letA mutant. Our data indicate that the response regulator LetA functions as a regulator of the stationary-phase stress response in L. pneumophila and is required for efficient replication within A. castellanii. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
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