Journal
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
Volume 14, Issue 8, Pages 4607-4610Publisher
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2013.14.8.4607
Keywords
Gastric cancer cells; immunosuppression; Treg cells; TGF-beta; IL-10
Categories
Ask authors/readers for more resources
The aim of this study was evaluated the prevalence of Treg cells in peripheral blood in patients with gastric cancer, and investigate the effect of gastric cancer cells on their differentiation. ELISA was employed to assess the concentrations of TGF-beta and IL-10 in gastric cancer patients' serum. Then, mouse gastric cancer cells were co-cultured with T lymphocytes or T lymphocytes + anti-TGF-beta. Flow cytometric analysis and RT-PCR were then performed to detect Treg cells and TGF-beta and IL-10 expression in gastric cancer cells. Our data showed that the expression of TGF-beta and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors. The population of Treg cells and the expression levels of TGF-beta and IL-10 in the co-culture group were much higher than in the control group (18.6% vs 9.5%) (P<0.05). Moreover, the population of Treg cells and the expression levels of TGF-beta and IL-10 in the co-culture systerm were clearly decreased after addition of anti-TGF-beta (7.7% vs 19.6%) (P<0.01). In conclusion, gastric cancer cells may induce Treg cell differentiation through TGF-beta, and further promote immunosuppression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available