Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 6, Pages 2051-2058Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.6.2051-2058.2003
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The promoter of the Streptococcus pneumoniae putative fuculose kinase gene (fcsK), the first gene of a novel fucose utilization operon, is induced by fucose and repressed by glucose or sucrose. When the streptococcal polypeptide deformylase (PDF) gene (def1, encoding PDF) was placed under the control of P-fcsK, fucose-dependent growth of the S. pneumoniae (P-fcsK::defl) strain was observed, confirming the essential nature of PDF in this organism. The mode of antibacterial action of actinonin, a known PDF inhibitor, was also confirmed with this strain. The endogenous fuculose kinase promoter is a tightly regulated, titratable promoter which will be useful for target validation and for confirmation of the mode of action of novel antibacterial drugs in S. pneumoniae.
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