Amelioration of chilling stress by triadimefon in cucumber seedlings

Cucumber ( Cucumis satvus L.) seeds were imbibed in distilled water ( control) and 10 mg l-(1) triadimefon ( TDM) for 10 h and then grown in a plant growth chamber with a light/ dark temperature of 28/20 degreesC and a photoperiod of 14 h with a light intensity of 60 mumol m(-2) s(-1). 14- day- old seedlings were exposed to chilling stress with a light/ dark temperature of 6/3 degreesC for 4 d. TDM improved the growth rate of cucumber seedling subjected to chilling stress and increased photosynthetic pigments contents and relative water content compared with the control at the end of chilling stress. Chilling stress decreased protein content and the activities of SOD, CAT and POD, but it increased proline, H2O2 and MDA accumulation, and relative electrical conductivity. TDM ameliorated the injury caused by chilling stress by preventing decreases in protein content and the activities of SOD, CAT and POD and by inhibiting increases in proline, H2O2 and MDA contents, and relative electrical conductivity, which suggested that TDM ameliorated the negative effect of chilling stress.