Journal
BIOPOLYMERS
Volume 68, Issue 3, Pages 286-299Publisher
WILEY
DOI: 10.1002/bip.10244
Keywords
computer simulations; DNA polymerases; replication fidelity; free energy perturbation; linear response approximation; empirical valence bond; mismatched base pairs
Categories
Funding
- NIGMS NIH HHS [GM21422, GM24492] Funding Source: Medline
Ask authors/readers for more resources
Computer simulations can provide in principle quantitative correlation between the structures of DNA polymerases and the replication fidelity. This paper describes our progress in this direction. Using several theoretical approaches, including the free energy perturbation (FEP), linear response approximation (LRA), and the empirical valence bond (EVB) methods, we examined the stability of several mismatched base pairs in DNA duplex in aqueous solution, the contribution of binding energy to the fidelity of DNA polymerases beta and T7, and the mechanism and energetics of the polymerization reaction catalyzed by T7 DNA polymerase. (C) 2003 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available