Journal
NATURE MEDICINE
Volume 9, Issue 3, Pages 357-362Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nm835
Keywords
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Funding
- NHLBI NIH HHS [R01 HL57665, HL64793, HL61371] Funding Source: Medline
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Small polybasic peptides derived from the transduction domains of certain proteins, such as the third alpha-helix of the Antennapedia (Antp) homeodomain, can cross the cell membrane through a receptor-independent mechanism. These cell-permeable molecules have been used as 'Trojan horses' to introduce biologically active cargo molecules such as DNA, peptides or proteins into cells. Using these cell-permeable peptides, we have developed an efficient and simple method to increase virally mediated gene delivery and protein expression in vitro and in vivo. Here, we show that cell-permeable peptides increase viral cell entry, improve gene expression at reduced titers of virus and improve efficacy of therapeutically relevant genes in vivo.
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