Journal
JOURNAL OF VIROLOGY
Volume 77, Issue 6, Pages 3838-3845Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.77.6.3838-3845.2003
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Funding
- NIAID NIH HHS [R21 AI47216] Funding Source: Medline
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Integrase can insert retroviral DNA into almost any site in cellular DNA; however, target site preferences are noted in vitro and in vivo. We recently demonstrated that amino acid 119, in the alpha2 helix of the central domain of the human immunodeficiency virus type 1 integrase, affected the choice of nonviral target DNA sites. We have now extended these findings to the integrases of a nonprimate lentivirus and a more distantly related alpharetrovirus. We found that substitutions at the analogous positions in visna virus integrase and Rous sarcoma virus integrase changed the target site preferences in five assays that monitor insertion into nonviral DNA. Thus, the importance of this protein residue in the selection of nonviral target DNA sites is likely to be a general property of retroviral integrases. Moreover, this amino acid might be part of the cellular DNA binding site on integrase proteins.
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