Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 51, Issue 3, Pages 401-404Publisher
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540305100315
Keywords
green fluorescent protein; transgenes; vapor fixation
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Green fluorescent protein (GFP) and its variants, such as enhanced GFP (EGFP), have been introduced into mammalian cells by transgenes, e.g., to distinguish donor from host cells after transplantation. Free GFP is extremely soluble and leaks out from liquid-covered cryostat sections so that fixation of whole organs before sectioning has been mandatory. This precludes the analysis of serial sections with respect to fixation-sensitive enzyme activities and antigens. We describe here a vapor fixation for sections from unfixed cryostat blocks of tissue that allows unrestricted enzyme and immunohistochemistry on adjacent sections, as demonstrated for cross-striated muscle and other tissues from EGFP transgenic green mice and for a transplantation experiment.
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