Journal
TALANTA
Volume 59, Issue 3, Pages 493-499Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(02)00532-5
Keywords
cyclodextrin; procaine hydrochloride; binding constant; capillary electrophoresis
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The apparent electrophoretic mobilities of procaine hydrochloride (mu(i)) in a series of concentration of beta-cyclodextrin were measured directly by capillary electrophoresis technology. A new mathematical treatment method is proposed, which based on the fact that the molar ratio of the inclusion complex was 1:1 established by spectrophotometry. Using the proposed method, the binding constant of the inclusion complex of procaine hydrochloride with beta-cyclodextrin can be obtained easily. The determination result was in correspondence with those of the spectrophotometric and fluorescence methods. (C) 2002 Elsevier Science B.V. All rights reserved.
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