Journal
TALANTA
Volume 59, Issue 3, Pages 453-459Publisher
ELSEVIER
DOI: 10.1016/S0039-9140(02)00535-0
Keywords
capillary electrophoresis; electrochemiluminescence detection; diphenhydramine; rabbit plasma; rabbit urine
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Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection in a capillary electrophoresis separation system was used for the determination of diphenhydramine. In this study, platinum disk electrode (300 mum in diameter) was used as a working electrode and the influence of applied potential and buffer conditions were investigated. Under optimal conditions: 1.2 V applied potential, pH 8.50, 15 kV separation voltage and 10 mmol l(-1) running buffer, the calibration curve of diphenhydramine was linear over the range of 4 x 10(-8) to 1 x 10(-5) Mol l(-1). This technique gave satisfactory precision, and relative standard deviations of migration times and chemiluminescence peak intensities were less than 1 and 6%, respectively. The technique was applied to animal studies for determination of diphenhydramine extracted from rabbit plasma and urine samples, and the extraction efficiency were between 92 and 98.5%. (C) 2002 Elsevier Science B.V. All rights reserved.
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