Journal
ASIAN JOURNAL OF CHEMISTRY
Volume 26, Issue 12, Pages 3541-3544Publisher
ASIAN JOURNAL OF CHEMISTRY
DOI: 10.14233/ajchem.2014.16198
Keywords
Molecularly imprinted polymers; Solid-phase extraction; Hybrid composite materials; Milk; Tetracyclines
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Funding
- National Natural Science Foundation of China [21275053]
- Natural Science Foundation of Hebei Province [B2011201081]
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A novel internal-surface reversed-phase-molecularly imprinted polymer based on hybrid composite was developed for solid-phase extraction (SPE) of tetracyclines antibiotics from milk. The condition of loading, washing and eluting solvents in solid-phase extraction were investigated and evaluated by the recovery and retention of tetracyclines to obtain the optimum solid-phase extraction conditions. Under the optimal solid phase extraction conditions, three tetracyclines residues in milk were separated and detected by SPE-HPLC with better clean-up and enrichment. The mean recoveries of tetracycline antibiotics in milk were 85-106 % for tetracycline, 76-88 % for chlortetracycline and 90-94 % for doxycycline respectively, with relative standard deviations (RSDs) of 3.3-5.8%. The limits of detection (LOD, S/N = 3) and the limits of quantitation (LOQ, S/N = 10) of the proposed method were 10.4 and 34.7 mg kg(-1) for tetracycline, 13.8 and 46 mg kg(-1) for chlortetracycline and 7.5 and 25.1 mg kg(-1) for doxycycline, respectively.
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