Phytochemical Spectrum of Essential Oil of Paganum harmala by GC-MS and Antimicrobial Activity Using Sequential Solvents Fractions and Essential Oil

Present study was designed to evaluate the antimicrobial potential of essential oil and extracts of different parts of Paganum harmala. Methanolic extract was prepared and extracted further using different organic solvents n-hexane, chloroform, ethyl acetate, n-butanol and water. These extracts were then used for determining their antimicrobial potential.. The sample found conspicuous antimicrobial activity of different extracts. In addition, extracted essential oil of this plant using super critical fluid extraction show antimicrobial activity. In essential oil, 1,2-bis[1-(2-hydroxyethyl)-3;6-dizazhomodamantantydene-9]hydrazine (13.29 %), methanol, [4-(1,1-dimethylethyl)phenoxy], acetate (11.29 %), Uridine, 5-tr hexyl-idecafluoro (8.56%), Tetracosane (5.40%) and bis[phenylsulfonyl]-4-trichloromethylphenyl. chloromethane (5.29%) were the major constituents. Antimicrobial potential of P. harmala could be attributed to the compounds detected in essential oil.