A simple and rapid method for nuclear run-on transcription assays in plants

Nuclear run-on transcription assays allow researchers to determine if a gene is regulated at the transcriptional level. Existing methods to perform this procedure involve the application of a large variety of biochemical reagents, numerous steps, and time-consuming procedures. Here we report a method to perform this assay on plant tissue that involves a minimal number of reagents and steps, is time-efficient, and produces clean definitive results within 24 h.