Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 21, Issue 1, Pages 65-71Publisher
SPRINGER
DOI: 10.1007/BF02773398
Keywords
method; nuclear; plants; run-on; transcription
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Nuclear run-on transcription assays allow researchers to determine if a gene is regulated at the transcriptional level. Existing methods to perform this procedure involve the application of a large variety of biochemical reagents, numerous steps, and time-consuming procedures. Here we report a method to perform this assay on plant tissue that involves a minimal number of reagents and steps, is time-efficient, and produces clean definitive results within 24 h.
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