Journal
BRITISH JOURNAL OF HAEMATOLOGY
Volume 120, Issue 5, Pages 846-849Publisher
WILEY-BLACKWELL
DOI: 10.1046/j.1365-2141.2003.04217.x
Keywords
mesenchymal cells; acute lymphoblastic leukaemia; haematopoiesis
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Funding
- NCI NIH HHS [CA21765, CA58297] Funding Source: Medline
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To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34(+) haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.
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