Journal
EXPERIMENTAL PARASITOLOGY
Volume 103, Issue 3-4, Pages 143-151Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0014-4894(03)00097-3
Keywords
Leishmania infantum; cysteine proteinases type I and II; visceral leishmaniasis; dog; human
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In this study, the mature domains of type I (CPB) and type 11 (CPA) cysteine proteinases (CPs) of Leishmania infantum were expressed and their immunogenic properties defined using sera from active and recovered cases of human visceral leishmaniasis and sera from infected dogs. Immunoblotting and ELISA analysis indicated that a freeze/thaw extract of parasite antigens showed similar and intensive recognition in both active cases of human and dog sera but lower recognition in recovered human individuals. The total IgG of actively infected human sera was higher than in recovered cases when rCPs were used as antigen. In contrast to dog sera, both active and recovered human cases have higher recognition toward rCPB than rCPA. Furthermore, the asymptomatic dogs in contrast to the symptomatic cases exhibited specific lymphocyte proliferation to both crude antigens and rCPs. (C) 2003 Elsevier Science (USA). All rights reserved.
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