Journal
BLOOD
Volume 101, Issue 5, Pages 1677-1683Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2002-07-2348
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Funding
- NCRR NIH HHS [MO1RR00750] Funding Source: Medline
- NIAID NIH HHS [AI27863, AI46784, T32AI07367, AI31494] Funding Source: Medline
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The chemokine receptors (CCRs) CCR4 and CCR10, and the cutaneous lymphocyte antigen (CLA), have each been proposed as critical mediators of skin-specific T-H lymphocyte homing in mice and humans. CLA initiates skin homing by mediating E-selectin-dependent tethering and rolling within cutaneous venules, but the specific roles of CCR4 and CCR10 are unclear. We have generated an antihuman CCR10 monoclonal antibody (mAb; 1B5) to illuminate the individual contributions of these molecules. This mAb allows us to compare CCR10, CCR4, and CLA expression within human T-H populations. The mAb 1B5 recognizes functional CCR10 expression, as chemotactic responsiveness to cutaneous T-cell-attracting chemokine (CTACK)/CCL27 (a CCR10 ligand) parallels the staining of T-H subsets. We find CCR10 expressed by only a minority (approximately 30%) of bloodborne, skin-homing (CLA(+)/CCR4(+)) T-H cells. However, essentially all members of the relatively small effector (CLA(+)/ CCR4(+)/CD27(-)/CCR7(-)) skin-homing T-H population express CCR10. Most skin-infiltrating lymphocytes in allergic delayed-type hypersensitivity (DTH) and bacterial chancroid skin lesions express both CCR4 and CLA, but only about 10% express CCR10. This suggests for the 2 models of T-H skin homing studied here that CCR10(+) T-H cells have no advantage over other CLA(+)/CCR4(+) T-H cells in homing to cutaneous sites. We conclude that the skin-homing T-H compartment is itself divided into distinct subpopulations, the smaller of which expresses both CCR4 and CCR10, and the larger of which expresses only CCR4. Thus, CCR10 is unlikely to be necessary for cutaneous homing of T-H cells in the models studied here. CCR10 may instead play a role in the movement of specialized effector cutaneous T-H cells to and/or within epidermal microenvironments.
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