Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 6, Pages 469-472Publisher
SPRINGER
DOI: 10.1023/A:1022612905134
Keywords
alginate; cytokinins; plant cell immobilization; roscovitine; tobacco
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Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N-6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N-6-Benzyladenine (1.25 muM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 muM) further increased cis-cytokinins, up to 2.2 nM.
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