Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 5, Pages 1730-1733Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.5.1730-1733.2003
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We studied the pattern of the cis-trans isomerization of unsaturated fatty acids in cells of Pseudomonas putida S12 grown in a medium supplemented with oleic acid which was deuterated at both of the C atoms of its double bond. Direct evidence that isomerization does not include a transient saturation of the double bond was obtained. In addition, analysis of the amino acid sequences of the seven known Cti proteins identified them as heme-containing proteins of the cytochrome c type.
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