Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 52, Issue 3, Pages 389-393Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(02)00210-5
Keywords
DNA extraction; DNA purification; PCR inhibition
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Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH4(SO4)(2) during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield. (C) 2002 Elsevier Science B.V. All rights reserved.
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