Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 5, Pages 2616-2621Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0537347100
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- NIAID NIH HHS [P01 AI045123, AI45123, R37 AI2416] Funding Source: Medline
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The lack of genetic means has been a serious limitation in studying mosquito immunity. We generated Relish-mediated immune deficiency (RMID) by transforming Aedes aegypti with the DeltaRel transgene driven by the vitellogenin (Vg) promoter using the pBac[3xP3-EGFP, afm] vector. A stable transformed line had a single copy of the Vg-DeltaRel transgene. The Vg-DeltaRel transgene expression was highly activated by blood feeding, and transgenic mosquitoes were extremely susceptible to the infection by Gram-negative bacteria. This RMID phenotype was characterized by severely reduced postinfection levels of antimicrobial peptides genes, defensin and cecropin. Crossing the RMID line with the wild-type strain produced the same RMID phenotype, indicating its dominant nature, whereas crossing with the Vg-def transgenic line, in which Defensin A was activated by blood feeding, restored the immunity to Enterobacter cloacae.
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