Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 125, Issue 9, Pages 2416-2425Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja026917a
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Funding
- NCI NIH HHS [R33 CA 89810] Funding Source: Medline
- NCRR NIH HHS [RR 00862] Funding Source: Medline
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A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.
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