Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 989, Issue 2, Pages 197-205Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)00119-5
Keywords
pressurized liquid extraction; molecular biomarkers; deep sea sediments; U-37(K ') index; alkenones
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Pressurized liquid extraction has been performed on a suite of deep-sea sediments to assess its capability as an extraction technique in the analysis of molecular biomarkers used in paleoceanography. Specific compounds assessed comprise long-chain alkenones, n-alkanes, n-alcohols and, additionally, one diol and one keto-ol. These have been extracted by both pressurized liquid extraction and ultrasonication for comparison. One key result is that the U-37(K') index (based on the degree of unsaturation of the alkenones and used as a paleothermometer in paleoceanography) remains intact after both extraction techniques. In terms of biomarker concentrations, which are often used to qualitatively assess changes in marine productivity and/or terrigenous inputs, pressurized liquid extraction is substantially more efficient than ultrasonication, providing higher amounts of extracted constituents, particularly for polar compounds. (C) 2003 Elsevier Science B.V. All rights reserved.
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