Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 989, Issue 2, Pages 257-264Publisher
ELSEVIER
DOI: 10.1016/S0021-9673(03)00081-5
Keywords
cereals; sample preparation; derivatization, LC; food analysis; anthroyinitriles; toxins
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1-Anthroylnitrile (1-AN) has been shown to be an efficient labelling reagent for the determination of T-2 toxin (T-2) by Anthroylnitrile (I-AN) has been shown to be an efficient labelling reagent for the determination of T-2 toxin (T-2) by high-performance liquid chromatography (HPLC)-fluorescence detection. This reaction has been used to develop a sensitive, reproducible and accurate method for the determination of T-2 in wheat, corn, barley, oats, rice and sorghum. The method uses immunoaffinity columns containing antibodies specific for T-2 for extract clean-up, pre-column derivatization with I-AN and HPLC with fluorescence detection for toxin determination. Ground cereal samples were extracted with methanol-water (80:20, v/v), the extracts were purified by immunoaffinity columns and the toxin was quantified by reversed-phase HPLC with fluorometric detection (excitation wavelength 381 nm, emission wavelength 470 nm) after derivatization with I-AN. Recoveries from the different cereals spiked with T-2 at levels ranging from 0.05 to 1.5 mug/g were from 80 to 99%, with relative standard deviations of less than 6%. The limit of detection was 0.005 mug/g, based on a signal-to-noise ratio of 3:1. (C) 2003 Elsevier Science B.V. All rights reserved.
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