Journal
EMBO JOURNAL
Volume 22, Issue 6, Pages 1389-1397Publisher
OXFORD UNIV PRESS
DOI: 10.1093/emboj/cdg136
Keywords
gene conversion; meiotic drive; meiotic recombination hotspot; mouse MHC E-beta gene
Categories
Ask authors/readers for more resources
Meiotic crossovers detected by pedigree analysis in the mouse MHC cluster into hotspots. To explore the properties of hotspots, we subjected the class II E-beta gene to high-resolution sperm crossover analysis. We confirm the presence of a highly localized hotspot 1.0-1.6 kb wide in the second intron of E-beta and show that it is flanked by DNA which is almost completely recombinationally inert. Mice heterozygous for haplotype s and another MHC haplotype show major haplotype-dependant variation in crossover rate but always the same hotspot, even in crosses including the highly diverged p haplotype. Crossovers in reciprocal orientations occur at similar rates but show different distributions across the hotspot, with the position of centre points in the two orientations shifted on average by 400 bp. This asymmetry results in crossover products showing biased gene conversion in favour of hotspot markers from the non-initiating haplotype, and supports the double-strand break repair model of recombination, with haplotype s as the most efficient crossover initiator. The detailed behaviour of the E-beta hotspot, including evidence for highly localized recombination initiation, is strikingly similar to human hotspots.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available