Journal
JOURNAL OF CHEMICAL PHYSICS
Volume 118, Issue 12, Pages 5279-5282Publisher
AMER INST PHYSICS
DOI: 10.1063/1.1562194
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We present a simple and straightforward method for determining absolute spatial orientations of transition dipole moments of single fluorescent molecules. Far-field polarization microscopy provides angles of the dipole moments projected in the plane of the sample. Optical field near total internal reflection surfaces has a strong component perpendicular to the sample and, for a given in-plane angle, provides unambiguous orientation of the molecular dipole moment. Experimentally, both excitation modes are alternated to monitor real-time conformational dynamics of tetramethylrhodamine molecules covalently attached to a quartz substrate. (C) 2003 American Institute of Physics.
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