Journal
FEBS LETTERS
Volume 539, Issue 1-3, Pages 24-28Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00178-9
Keywords
ribosome display; in vitro; protein folding
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Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to. perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) increased sensitivity to proteolysis and (2) greater exposure of hydrophobic area. By targeting these properties, we show that compactly folded and soluble proteins can be enriched over insoluble and random coil proteins. This approach may be especially useful for selection and evolution of folded proteins from random sequence libraries. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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