A carbazole derivative synthesis for stabilizing the quadruplex structure

A new molecule of 3,6-Bis(1-methyl-4-vinylpyridium iodine)carbazole (BMVC) was synthesized for stabilizing the quadruplex structure of human telomeric sequence of d(T(2)AG(3))(4) in vitro. Mixing BMVC with the DNA can raise the melting temperature of the d(T(2)AG(3))(4) by similar to13 degreesC, implying that BMVC could be a useful telomerase inhibitor. In addition, the fluorescence of the BMVC increased significantly upon interacting with the d(T(2)AG(3))(4) which may be useful as a G-quadruplex specific marker.