Journal
MOLECULAR CELL
Volume 11, Issue 4, Pages 1101-1108Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(03)00134-5
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Funding
- NIGMS NIH HHS [R01 GM37828] Funding Source: Medline
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We have employed a hidden Markov model (HMM) based on known cAMP responsive elements to search for putative CREB target genes. The best scoring sites were positionally conserved between mouse and human orthologs, suggesting that this parameter can be used to enrich for true CREB targets. Target validation experiments revealed a core promoter requirement for transcriptional induction via CREB; TATA-less promoters were unresponsive to cAMP compared to TATA-containing genes, despite comparable binding of CREB to both sets of genes in vivo. Indeed, insertion of a TATA box motif rescued cAMP responsiveness on a TATA-less promoter. These results illustrate a mechanism by which subsets of target genes for a transcription factor are differentially regulated depending on core promoter configuration.
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