Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 73, Issue 1, Pages 53-56Publisher
SPRINGER
DOI: 10.1023/A:1022619523726
Keywords
Medicago; proline; salt stress; sodium chloride
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In this study, two cultivars of Medicago sativa (cv. Yazdi and cv. Hamedani) were used for callus production. Calluses were transferred to MS medium containing 0, 30, 60, 90, and 120 mM NaCl and 0, 5, 10 mM proline. After 4-5 weeks dry weight and intracellular free proline of the calluses were measured. The growth of callus in both cultivars decreased with increasing salt concentration. Addition of exogenous proline to the culture medium increased the dry weight and free proline content of callus. The difference between control and treated calluses with 10 mM exogenous proline in the medium was significant. The data obtained from experiments indicated that the responses of two Medicago cultivars was genotype dependent.
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