Journal
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES
Volume 1, Issue 2, Pages 291-303Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/15406580360545107
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A study comparing five different cAMP detection technologies in terms of sensitivity, robustness, and feasibility for HTS is presented. In this report, the following methods are described: a nonhomogeneous DELFIA(R), and the homogeneous methods based on time-resolved fluorescence (HTRF(R)), luminescent singlet oxygen channeling or ALPHAScreen(R), FP, and high-affinity enzyme complementation. DELFIA had the highest sensitivity, whereas ALPHAScreen and HTRF shared several advantages, including high sensitivity, broad dynamic range, and minimal reagent addition steps. For G(s)-coupled antagonist screens, we found HTRF and ALPHAScreen the more sensitive and HTS-compatible techniques.
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