Journal
POSTHARVEST BIOLOGY AND TECHNOLOGY
Volume 28, Issue 1, Pages 39-46Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-5214(02)00143-6
Keywords
broccoli; cell localization; chlorophyll; chlorophyll-degrading peroxidase; heat treatment
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Cell localization of chlorophyll (Chl)-degrading peroxidase and the effects of heat treatment on the activity were determined to elucidate the involvement of the enzyme with yellowing in stored broccoli. Subcellular fractions and intact chloroplasts were prepared by differential and Percoll gradient centrifugation, respectively. In fresh broccoli, only low levels of Chl-degrading peroxidase activity were located in the intact chloroplast, and the highest activity was present in the cytosolic fraction. The Chl content of broccoli without heat treatment decreased greatly after 4 days storage at 15 degreesC, whereas the content in broccoli treated at 50 degreesC for 2 h showed almost no change during storage. In microsomal and cytosolic fractions, the activity of C-2 (R-f = 0.47) isoperoxidase, which is involved in Chl degradation, increased greatly with floret yellowing. The Chl-degrading peroxidase activities in microsomal and cytosolic fractions also increased strongly after 4 days of storage. In addition, the Chl-degrading peroxidase activity as well as C-2 isoperoxidase activity was suppressed by heat treatment. These results indicate that heat treatments may be effective in inhibiting senescence in part by suppressing the enhancement of Chl-degrading peroxidase activity in the microsomes and the cytosol. (C) 2002 Elsevier Science B.V. All rights reserved.
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