Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 275, Issue 1-2, Pages 113-121Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(03)00009-7
Keywords
flow cytometry; cell cycle analysis; DNA staining; immunofluorescence
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Funding
- NCI NIH HHS [CA08748] Funding Source: Medline
- NIAID NIH HHS [AI33940] Funding Source: Medline
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In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform. (C) 2003 Elsevier Science B.V. All rights reserved.
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