Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 41, Issue 4, Pages 1763-1765Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.41.4.1763-1765.2003
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DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerise. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.
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