Journal
JOURNAL OF FOOD SCIENCE
Volume 68, Issue 3, Pages 861-865Publisher
WILEY
DOI: 10.1111/j.1365-2621.2003.tb08256.x
Keywords
alpha-carotene; free radical scavenger; DPPH; electron donation mechanism; NMR
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The free radical scavenging ability of beta-carotene was studied using free radical 2,2-diphenyl-1- picryl-hydrazyl (DPPH). As the concentration of beta-carotene in acetone increased, the absorbance of DPPH at 517 nm decreased, which indicates that beta-carotene scavenges free radicals. It was shown that beta-carotene does not work as an antioxidant in soybean oil. The NMR spectrum of DPPH with BHA in CDCl3 showed a new peak at 5.0(d) was formed and the peak at 6.6(d) from BHA was split into 2 peaks, which may be due to the hydrogen atom donation from BHA to DPPH. The NMR spectra of DPPH with beta-carotene did not change during reaction and no peak was formed.
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