Journal
PROTEOMICS
Volume 3, Issue 4, Pages 558-561Publisher
WILEY
DOI: 10.1002/pmic.200390069
Keywords
avidin; biotin; EnVision (TM); kidney; streptavidin; Western blotting
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Western blotting remains a central technique in confirming identities of proteins, their quantitation and analysis of various isoforms. The biotin-avidin/streptavidin system is often used as an amplification step to increase sensitivity but in some tissues such as kidney, nonspecific interactions may be a problem due to high levels of endogenous biotin-containing proteins. The EnVision(TM)+ system, developed for immunohistochemical applications, relies on binding of a polymeric conjugate consisting of up to 100 peroxidase molecules and 20 secondary antibody molecules linked directly to an activated dextran backbone, to the primary antibody. This study demonstrates that it is also a viable and sensitive alternative detection system in Western blotting applications.
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