Journal
CELLULAR IMMUNOLOGY
Volume 222, Issue 2, Pages 126-133Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0008-8749(03)00128-X
Keywords
dendritic cells; cross-presentation; ovalbumin; immunization; interleukin 2; cytotoxic T lymphocyte; ELISPOT
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Funding
- PHS HHS [A149213-02] Funding Source: Medline
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Undifferentiated and differentiated dendritic cells (uDC and dDC, respectively), derived from the bone marrow, were studied in vitro and in vivo. Ovalbumin (OVA) and two OVA-derived peptides binding to H-2K(b) and 1-A(b), respectively, were used. Two IL-2 secreting T cell hybridomas specific for the OVA-derived epitopes were used in the in vitro read-out. The ability to cross-present the H-2K(b) binding OVA(2577-264)-peptide (SIINFEKL) was restricted to dDC, which express CD11c(+), CD86(+), and MHC-II+. In vitro, the antigenicity of SIINFEKL-loaded DC declined at a slower rate than that of OVA-pulsed DC. Moreover, SIINFEKL-loaded DC were up to 50 times more efficient than DC-pulsed with OVA-protein for generation of an H-2K(b)-restricted response. Immunization of mice with SIINFEKL-loaded DC resulted in a much stronger H-2K(b)-restricted response than immunization with OVA-pulsed DC. These data might have important implications for the choice of antigen source in the design of DC-based vaccines. (C) 2003 Elsevier Science (USA). All rights reserved.
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