Journal
FEMS YEAST RESEARCH
Volume 3, Issue 2, Pages 185-189Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1567-1356(02)00184-8
Keywords
L-arabinose; pentose fermentation; L-arabinitol 4-dehydrogenase; L-xylulose reductase; Saccharomyces cerevisiae
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The fungal pathway for L-arabinose catabolism converts c-arabinose to D-xylulose 5-phosphate in five steps. The intermediates are, in this order: L-arabinitol, L-xylulose, xylitol and D-xylulose. Only some of the genes for the corresponding enzymes were known. We have recently identified the two missing genes for L-arabinitol 4-dehydrogenase and L-xylulose reductase and shown that overexpression of all the genes of the pathway in Saccharomyces cerevisiae enables growth on L-arabinose. Under anaerobic conditions ethanol is produced from L-arabinose, but at a very low rate. The reasons for the low rate of L-arabinose fermentation are discussed. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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