Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 7, Issue 2, Pages 165-170Publisher
WILEY
DOI: 10.1111/j.1582-4934.2003.tb00215.x
Keywords
astrocytes; blood-brain barrier; brain capillary endothelial cells; cortical neurons; cocultures; occludin; tight junctions
Categories
Ask authors/readers for more resources
Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary endothelial cells in culture. In particular, we found that, in the presence of astrocytes, the neuron-induced synthesis and localization of occludin is precocious as compared to cells cocultured with neurons only.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available