Journal
CELL DEATH AND DIFFERENTIATION
Volume 10, Issue 4, Pages 451-460Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4401180
Keywords
apoptosis; p53; Bax; cytochrome c; Noxa
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Funding
- NCI NIH HHS [CA69381] Funding Source: Medline
- NIAID NIH HHS [AI40646] Funding Source: Medline
- NIGMS NIH HHS [GM52735] Funding Source: Medline
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The mechanism of p53-dependent apoptosis is still only partly defined. Using early-passage embryonic fibroblasts (MEF) from wild-type (wt), p53(-/-) and bax(-/-) mice, we observe a p53-dependent translocation of Bax to the mitochondria and a release of mitochondrial Cytochrome c during stress-induced apoptosis. These events proceed independent of zVAD-inhibitable caspase activation, are not prevented by dominant negative FADD (DN-FADD), but are negatively regulated by Mdm-2. Bcl-x(L) expression prevents the release of mitochondrial Cytochrome c and apoptosis, but not Bax translocation. At a single-cell level, enforced expression of p53 is sufficient to induce Bax translocation and Cytochrome c release. Real-time RT-PCR analysis reveals a significant induction of RNA expression of Noxa and Bax in p53(+/+), but not in p53(-/-) MEF. Noxa protein expression becomes detectable prior to Bax translocation, and downregulation of endogenous Noxa by RNA interference protects wt MEF against p53-dependent apoptosis. Hence, in oncogene-expressing MEF p53 induces apoptosis by BH3 protein-dependent caspase activation.
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