Journal
WATER RESEARCH
Volume 37, Issue 8, Pages 1972-1975Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0043-1354(02)00533-X
Keywords
17 beta-estradiol; ethinylestradiol; estrogenic activity; manganese peroxidase; laccase-mediator
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We investigated whether manganese peroxidase (MnP) and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as mediator can remove the estrogenic activities of the steroidal hormones 17beta-estradiol (E-2) and ethinylestradiol (EE2). Using the yeast two-hybrid assay system, we confirmed that the estrogenic activities of E-2 and EE2 are much higher than those of bisphenol A and nonylphenol. Greater than 80% of the estrogenic activities of E-2 and EE2 were removed following I-h treatment with MnP or the laccase-HBT system; extending the treatment time to 8 h removed the remaining estrogenic activity of both steroidal hormones. HPLC analysis demonstrated that E-2 and EE2 had disappeared almost completely in the reaction mixture after a 1-h treatment. These results strongly suggest that these ligninolytic enzymes are effective in removing the estrogenic activities of E-2 and EE2. (C) 2002 Elsevier Science Ltd. All rights reserved.
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