Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 53, Issue 1, Pages 131-135Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(02)00232-4
Keywords
fluorescence spectroscopy; Nile red; medium-chain-length; polyhydroxyalkanoate; short-chain-length
Categories
Ask authors/readers for more resources
An approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells. (C) 2002 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available