Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 20, Issue 5, Pages 693-701Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2003.10506886
Keywords
telomeric DNA; nanowire structure; quadruplex; monovalent cations; circular dichroism; atomic force microscopy
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The telomeric DNA oligomers, d(TTAGGG)(n), where n=1, 2, 4, could self-associate into the multi-stranded structures in appropriate condition and exhibited a different CD spectra. The present of Na+ was more advantage to facilitate the formation of anti-parallel conformation, but the present of K+ enhanced their thermal stability. Spectroscopic analysis of 3, 3'-diethyloxadicarbocyanine (DODC) showed the formation of hairpin quadruplex structures for d(TTAGGG)(2) and d(TTAGGG)(4), but d(TTAGGG) could not. The four-stranded tetraplexes and branched nanowire formed in the present of K+ or Na+ alone were observed by atomic force microscopy (AFM). The ability to self-assemble of d(TTAGGG)(n), into four-stranded tetraplexes and nanowires depends strongly on the number of repeating units and ionic environment. A model to explain how these structures formed is proposed.
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