Journal
ARTIFICIAL ORGANS
Volume 32, Issue 12, Pages 982-989Publisher
WILEY
DOI: 10.1111/j.1525-1594.2008.00660.x
Keywords
Tissue engineering; Dual-pore PLLA scaffold; Acrylic acid grafting; RGD immobilization; Chondrocyte
Categories
Funding
- Ministry of Science and Technology, Korea [2E19220, 2E19362]
Ask authors/readers for more resources
Interactions between cell and polymer surface have great implications in tissue engineering. In this study, chondrocyte proliferation and matrix production were examined using porous poly(l-lactide) (PLLA) scaffolds that have different surface characteristics. PLLA scaffolds were prepared using a gas-foaming method, and subjected to surface modifications through plasma treatment and subsequent in situ grafting of hydrophilic acrylic acid (AA). To immobilize peptide ligands, the AA-grafted PLLA scaffolds (PLLA-PAA) were further reacted with either Gly-Arg-Asp-Gly (GRDG) or Gly-Arg-Gly-Asp GRGD) to produce PLLA-PAA-GRDG or PLLA-PAA-GRGD scaffold, respectively. The average porosities of the scaffolds were more than 90%, and their pore sizes ranged from 200 similar to 300 to 10 similar to 50 mu m for large and small pores, respectively. The concentrations of each bound component were 2.14 X 10(-4) mmol/cm(2) for AA, 1.87 nmol/g for GRDG, and 1.68 nmol/g for GRGD. When chondrocytes were seeded onto the different PLLA scaffolds, cell adhesion and proliferation were highly affected as the substrate types vary. The RGD-immobilized scaffolds resulted in higher cellularity and better accumulation of total glycosaminoglycan than the others. Histological staining of Safranin O showed that the deposited extracellular matrix was more intense and widely distributed in the PLLA-PAA-GRGD scaffold. The present data suggest that immobilization of RGD peptide on the AA-grafted PLLA scaffold can be an effective tool for chondrocyte attachment and proliferation, and that it may also be helpful to facilitate cartilaginous tissue formation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available